2021-06-15 13:52:56  招生问答

本期文章:《自然》:Online/在线发表

科学家揭示毛囊干细胞的起源


科学家揭示毛囊干细胞的起源

日本理化学研究所Hironobu Fujiwara课题组揭示毛囊干细胞的起源。2021年6月9日,《自然》杂志在线发表了这一最新研究成果。

科学家揭示毛囊干细胞的起源

通过结合独立于标记物的长期3D实时成像和单细胞转录组学,研究人员捕捉了小鼠毛囊整个上皮在发育过程中的动态谱系进展和转录组变化。研究人员发现,不同上皮谱系的前体在头发基板的基底层中以二维同心方式排列。每个同心环获得独特的转录组并延伸形成纵向排列的3D圆柱形隔室。即将充满的干细胞来自基板基底层的外围环,而不是上基底细胞。基板细胞的命运由细胞位置决定,而不是由细胞分裂的方向决定。

研究人员还确定了13个基因簇:这些簇的整体表达动态绘制了上皮谱系多样化的整个转录图谱,并与细胞谱系数据一致。将这些发现与之前关于昆虫附肢发育的工作相结合 ,研究人员描述了一个“望远镜模型”,这是一种用于发育外胚层器官的通用模型,其中基板中的2D同心区向外伸缩形成3D纵向对齐的圆柱形隔室。

据介绍,组织干细胞是通过器官特异性形态发生事件从一群胚胎祖细胞中产生的。尽管组织干细胞是器官稳态和再生的核心,但它们在发育过程中如何被诱导仍不清楚,主要是因为缺乏专门标记预期干细胞的标记物。

附:英文原文

Title: Tracing the origin of hair follicle stem cells

Author: Ritsuko Morita, Noriko Sanzen, Hiroko Sasaki, Tetsutaro Hayashi, Mana Umeda, Mika Yoshimura, Takaki Yamamoto, Tatsuo Shibata, Takaya Abe, Hiroshi Kiyonari, Yasuhide Furuta, Itoshi Nikaido, Hironobu Fujiwara

Issue&Volume: 2021-06-09

Abstract: Tissue stem cells are generated from a population of embryonic progenitors through organ-specific morphogenetic events1,2. Although tissue stem cells are central to organ homeostasis and regeneration, it remains unclear how they are induced during development, mainly because of the lack of markers that exclusively label prospective stem cells. Here we combine marker-independent long-term 3D live imaging and single-cell transcriptomics to capture a dynamic lineage progression and transcriptome changes in the entire epithelium of the mouse hair follicle as it develops. We found that the precursors of different epithelial lineages were aligned in a 2D concentric manner in the basal layer of the hair placode. Each concentric ring acquired unique transcriptomes and extended to form longitudinally aligned, 3D cylindrical compartments. Prospective bulge stem cells were derived from the peripheral ring of the placode basal layer, but not from suprabasal cells (as was previously suggested3). The fate of placode cells is determined by the cell position, rather than by the orientation of cell division. We also identified 13 gene clusters: the ensemble expression dynamics of these clusters drew the entire transcriptional landscape of epithelial lineage diversification, consistent with cell lineage data. Combining these findings with previous work on the development of appendages in insects4,5, we describe the ‘telescope model’, a generalized model for the development of ectodermal organs in which 2D concentric zones in the placode telescope out to form 3D longitudinally aligned cylindrical compartments.

DOI: 10.1038/s41586-021-03638-5

Source: https://www.nature.com/articles/s41586-021-03638-5

期刊信息

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07 官方网址: 投稿链接:

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